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pgl4 10 vector  (TaKaRa)


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    TaKaRa pgl4 10 vector
    Pgl4 10 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 615 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgl4 10 vector/product/TaKaRa
    Average 96 stars, based on 615 article reviews
    pgl4 10 vector - by Bioz Stars, 2026-02
    96/100 stars

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    pHDAC5 MUT shows a significantly increased luciferase activity compared to pHDAC5 WT . Dual luciferase reporter assays performed for pGL4.10- HDAC5 WT and pGL4.10- HDAC5 MUT reporter constructs co-transfected with pSNAI-2 or pTCF4 or no transcription factor (TF) into HEK293T cells. Luciferase activity was measured at four different time points ( a – d ) and normalized to the empty pGL4.10 reporter vector. Each bar represents the mean of three independent experiments with standard deviation. pHDAC5 MUT shows a significantly increased luciferase activity compared to pHDAC5 WT . Co-transfection of pTCF4 further enhanced the promoter activity of pHDAC5 MUT significantly. *— p < 0.05, **— p < 0.01, ***— p < 0.001, ****— p < 0.0001.

    Journal: International Journal of Molecular Sciences

    Article Title: Whole Exome Sequencing Identifies APCDD1 and HDAC5 Genes as Potentially Cancer Predisposing in Familial Colorectal Cancer

    doi: 10.3390/ijms22041837

    Figure Lengend Snippet: pHDAC5 MUT shows a significantly increased luciferase activity compared to pHDAC5 WT . Dual luciferase reporter assays performed for pGL4.10- HDAC5 WT and pGL4.10- HDAC5 MUT reporter constructs co-transfected with pSNAI-2 or pTCF4 or no transcription factor (TF) into HEK293T cells. Luciferase activity was measured at four different time points ( a – d ) and normalized to the empty pGL4.10 reporter vector. Each bar represents the mean of three independent experiments with standard deviation. pHDAC5 MUT shows a significantly increased luciferase activity compared to pHDAC5 WT . Co-transfection of pTCF4 further enhanced the promoter activity of pHDAC5 MUT significantly. *— p < 0.05, **— p < 0.01, ***— p < 0.001, ****— p < 0.0001.

    Article Snippet: Ligation of the digested HDAC5 gene insert (1099 bp) and pGL4.10 vector (4210 bp) was done by using Quick Ligation™ Kit (New England BioLabs, #M2200S).

    Techniques: Luciferase, Activity Assay, Construct, Transfection, Plasmid Preparation, Standard Deviation, Cotransfection